Uterine expression of sodium/potassium/calcium exchanger 3 and its regulation by sex‐steroid hormones during the estrous cycle of rats
Identifieur interne : 002670 ( Main/Exploration ); précédent : 002669; suivant : 002671Uterine expression of sodium/potassium/calcium exchanger 3 and its regulation by sex‐steroid hormones during the estrous cycle of rats
Auteurs : Hyun Yang [Corée du Sud] ; Yeong-Min Yoo [Corée du Sud] ; Eui-Man Jung [Corée du Sud] ; Kyung-Chul Choi [Corée du Sud] ; Eui-Bae Jeung [Corée du Sud]Source :
- Molecular Reproduction and Development [ 1040-452X ] ; 2010-11.
English descriptors
- Teeft :
- Body weight, Calcium ions, Diestrus, Dong, Embryo, Embryo implantation, Estrogen, Estrous, Estrous cycle, Estrus, Estrus cycle, Exchanger, Expression patterns, Female rats, Glandular epithelium, Immature rats, Implantation, Jeung, Krisinger, Mrna, Nckx3, Nckx3 expression, Nckx3 mrna, Nckx3 mrna expression, Nckx3 protein, Negative control, Proestrus, Progesterone, Rat, Reprod, Salamonsen, Santa cruz, Smooth muscle, Steroid, Steroid hormones, Subcutaneous injection, Trpv6, Uterine, Uterine expression, Uterine expressions, Uterine function, Uterine nckx3, Uterine nckx3 mrna, Uterus.
Abstract
Plasma membrane sodium/calcium exchangers are an important component of intracellular calcium homeostasis and electrical conduction. NCKX3 (gene SLC24A3), a potassium‐dependent sodium‐/calcium exchanger, plays a critical role in the transport of one intracellular calcium and potassium ion across the cell membrane in exchange for four extracellular sodium ions. NCKX3 transcripts are most abundant in the brain and smooth muscle, but many other tissues, in particular, the uterus, aorta and intestine, also express this gene at lower levels. However, the expression and physiological roles of NCKX3 in the uterus of rats during the estrous cycle are unknown. Thus, we examined the uterine expression of NCKX3 mRNA and protein at different stages of the estrous cycle in mature and immature female rats in the absence or presence of the sex‐steroid hormones estrogen (E2) and progesterone (P4). During the estrous cycle, uterine expression of NCKX3 mRNA and protein was enhanced up to 4.0‐ and 2.5‐fold, respectively, at proestrus compared to during estrus and diestrus. To examine the effect of sex steroids on NCKX3 regulation in the uterus, immature female rats were treated with E2 (40 µg/kg body weight; BW), P4 (4 mg/kg BW), or E2 plus P4 for 3 days. The expression of NCKX3 mRNA and protein was induced by E2, whereas P4 antagonized E2‐induced NCKX3 expression. Subsequent immunohistochemical analysis revealed that uterine NCKX3 protein was abundantly localized in the cytoplasm of luminal and glandular epithelial cells throughout the estrous cycle. Taken together, these results indicate that uterine NCKX3 is abundantly expressed in the uterus and that its expression is regulated by the steroid hormones, E2 and P4. These findings suggest that NCKX3 may be involved in reproductive function during the estrous cycle in female rats. Mol. Reprod. Dev. 77:971–977, 2010. © 2010 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/mrd.21245
Affiliations:
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NCKX3 (gene SLC24A3), a potassium‐dependent sodium‐/calcium exchanger, plays a critical role in the transport of one intracellular calcium and potassium ion across the cell membrane in exchange for four extracellular sodium ions. NCKX3 transcripts are most abundant in the brain and smooth muscle, but many other tissues, in particular, the uterus, aorta and intestine, also express this gene at lower levels. However, the expression and physiological roles of NCKX3 in the uterus of rats during the estrous cycle are unknown. Thus, we examined the uterine expression of NCKX3 mRNA and protein at different stages of the estrous cycle in mature and immature female rats in the absence or presence of the sex‐steroid hormones estrogen (E2) and progesterone (P4). During the estrous cycle, uterine expression of NCKX3 mRNA and protein was enhanced up to 4.0‐ and 2.5‐fold, respectively, at proestrus compared to during estrus and diestrus. To examine the effect of sex steroids on NCKX3 regulation in the uterus, immature female rats were treated with E2 (40 µg/kg body weight; BW), P4 (4 mg/kg BW), or E2 plus P4 for 3 days. The expression of NCKX3 mRNA and protein was induced by E2, whereas P4 antagonized E2‐induced NCKX3 expression. Subsequent immunohistochemical analysis revealed that uterine NCKX3 protein was abundantly localized in the cytoplasm of luminal and glandular epithelial cells throughout the estrous cycle. Taken together, these results indicate that uterine NCKX3 is abundantly expressed in the uterus and that its expression is regulated by the steroid hormones, E2 and P4. These findings suggest that NCKX3 may be involved in reproductive function during the estrous cycle in female rats. Mol. Reprod. Dev. 77:971–977, 2010. © 2010 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Yang, Hyun" sort="Yang, Hyun" uniqKey="Yang H" first="Hyun" last="Yang">Hyun Yang</name></noRegion><name sortKey="Choi, Kyung Hul" sort="Choi, Kyung Hul" uniqKey="Choi K" first="Kyung-Chul" last="Choi">Kyung-Chul Choi</name><name sortKey="Jeung, Eui Ae" sort="Jeung, Eui Ae" uniqKey="Jeung E" first="Eui-Bae" last="Jeung">Eui-Bae Jeung</name><name sortKey="Jeung, Eui Ae" sort="Jeung, Eui Ae" uniqKey="Jeung E" first="Eui-Bae" last="Jeung">Eui-Bae Jeung</name><name sortKey="Jeung, Eui Ae" sort="Jeung, Eui Ae" uniqKey="Jeung E" first="Eui-Bae" last="Jeung">Eui-Bae Jeung</name><name sortKey="Jung, Eui An" sort="Jung, Eui An" uniqKey="Jung E" first="Eui-Man" last="Jung">Eui-Man Jung</name><name sortKey="Yoo, Yeong In" sort="Yoo, Yeong In" uniqKey="Yoo Y" first="Yeong-Min" last="Yoo">Yeong-Min Yoo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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